study is a significant milestone in the perpetual journey to education and the
best exposure to this would be a worthy platform to prove the skills and
knowledge that I have learnt. With due reverence to the department and the
university, I pen down a ‘statement of
purpose’. This statement is an endeavor to project my credentials as an
aspiring researcher, my achievements as a biotechnology graduate, and perusing post-graduation as a
bioinformaticians my interest in this doctoral program and my ambitions.
have always been a curious child, seeking reason and logic behind my
observations of the world around me, and of the explanations put forth by
others. I was drawn towards biology by my uncle who is a Doctor. He would
regularly take me to observe surgeries in his hospital and explain various
concepts to me and also heard about laboratory. Life science has driven my
curiosity, understanding it provides me a sense of fulfillment.
I was in my sixth
standard, when I learned about the biotechnology of in-vitro reproduction-
studying life so closely and even altering it to such an extent- had left me
dumbfounded. I realised that Biotechnology would be one such course that would
encompass everything. Thus choosing a subject for my career was an easy
decision for me.
To this end, I pursued an
Engineering degree in Biotechnology and learned how subjects like Biochemistry,
Molecular Biology & Genetics, Immunology, Genetic Engineering,
Bioinformatics and Computational Molecular Biology all join the dots of a vast
picture. Apart from the core subjects in Life Sciences, my course included
subjects like Engineering Physics, Engineering Chemistry, Engineering Mathematics,
C programming and Data Structures, Basic Electrical and Electronic Engineering,
Probability and Statistics, and Instrumentation and Process Control amongst
others. Since in research nothing takes precedence over practical prowess, I
volunteered during my semester breaks at private labs to gain expertise in
Molecular Biology techniques.
During my First year summer break
I went to MRD Life Science, Lucknow, India a private lab to gain hands on
training on molecular biology for 15 days. Now my curiosity is increases to
know more about labs works then in my second year winter break again I joined
MRD Life Science, Lucknow, India but now in different topic state of art in
Enzymology where I studied about
fermentation, Downstream processing, Protein estimation and dialysis. After
that in my third year of graduation during summer vacation I was selected for the summer training Program, in molecular biology unit,
Institute of Medical Sciences Banaras Hindu University, Varanasi India, in
the summer of 2014. Where I am
very fortunate to work under Dr.
Sunit K.Singh, molecular biology unit, Institute of Medical Sciences Banaras
Hindu University, Varanasi India which taught me molecular biology
techniques used to medical science.
The opportunity I was seeking came during fourth year of my
graduation when I approached Prof.M.Radhakrishna
Pillai, for an intership as a part of
my curriculum at the, Rajiv Gandhi Centre for biotechnology,
Kerala India.Under the
guidance of Prof.M.Radhakrishna Pillai I worked on “Cloning and Expression of
capsid protein West Nile Virus in mammalian cells”.
My colleague Pradip Fulamli
and I closely investigated the West Nile Virus and study about how capsid protein
plays a vital role in host body replication. This study comprising of
Bioinformatics analysis, wet-lab validation and the functional studies. With the
help of Prof.M.Radhakrishna I came to know about
how wet lab analysis of the research can be made easier and faster by using the
bioinformatics tools “biotechnology researcher as a bioinformatics”.
Now to homogenize computational & biology I
enrolling into a formal course on Master’s program in Bioinformatics. During the
1st year, I was introduced to the different subject where I developed a special
interest in understanding the complexity of a biological system and types of
data used and what are the different approaches on different biological
problems at different levels i.e on system heterogeneity in size & timescale.
With this interest, I joined under Dr.Nooruddin Khan, in which the laboratory
mainly deals with “Immunoinformatics approach to identify peptide based vaccine
against dengue virus”.
During my tenure at
Dr. Noor’s lab I focused on gaining experience on Metabolic adaptation to the
changing nutrient levels in the cellular microenvironment plays a decisive role
in the maintenance of homeostasis. My colleague Srikanth Battu and Gillipsie Minhas and I
closely investigated the “Amino Acid
Sensing via General Control Nonderepressible-2 Kinase and
Immunological Programming” was published in Frontier Immunology.
The time I spent working here introduced me to the real world
of research, which is awe-inspiring. I started to appreciate the
interdependence of dry and wet research groups and their collaboration could
mount ground breaking research in the field of Biology.
Although I have a long road ahead to reach my goal, my
journey so far has given me the confidence to seek the prestigious Research
Center for Molecular Medicine of the Austrian Academy of Sciences for my
Doctoral studies. I desire to closely work with the immunological research
groups at the Research Center for Molecular Medicine. Recent research on
employing High-throughput sequencing (HTS) of
immune repertoires has enabled the quantitative analysis of adaptive immune
responses and offers the potential to revolutionize research in lymphocyte
biology, vaccine profiling, and monoclonal antibody engineering. Advances in
sequencing technology coupled to an exponential decline in sequencing costs
have fueled the recent overwhelming interest in immune repertoire sequencing.
This, in turn, has sparked the development of numerous methods for bioinformatics
and statistics-driven interpretation and visualization of immune repertoires
I strongly look forward to the possibility that I will make a
successful career as a researcher and hope that the Research Center for
Molecular Medicine of the Austrian Academy of Sciences will help me in my endeavor.
Regards, Aman Mishra